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normal human colon mucosal epithelial cell line ncm460  (Procell Inc)

 
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    Procell Inc normal human colon mucosal epithelial cell line ncm460
    Normal Human Colon Mucosal Epithelial Cell Line Ncm460, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human colon mucosal epithelial cell line ncm460/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    normal human colon mucosal epithelial cell line ncm460 - by Bioz Stars, 2026-03
    90/100 stars

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    (A) IC 50 values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of <t>epithelial</t> and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E . Data from three independent experiments were graphed as the mean ± SD. * P < 0.05.
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    (A) IC 50 values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of epithelial and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E . Data from three independent experiments were graphed as the mean ± SD. * P < 0.05.

    Journal: Oncotarget

    Article Title: Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters

    doi: 10.18632/oncotarget.17548

    Figure Lengend Snippet: (A) IC 50 values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of epithelial and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E . Data from three independent experiments were graphed as the mean ± SD. * P < 0.05.

    Article Snippet: The normal human colon mucosal epithelial cell line (NCM460), the low invasive human colon adenocarcinoma cell line (HT-29), the highly invasive human colon cancer cells (HCT-8), HCT-8/V and Bel7402 hepatocarcinoma cell line were purchased from KeyGEN BioTECH (Nanjing, China).

    Techniques: Flow Cytometry, Western Blot, Control, Staining, Immunofluorescence, Expressing